Height Resolution of Antibody Spots Measured by Spinning-Disk Interferometry on the BioCD
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چکیده
منابع مشابه
Height Resolution of Antibody Spots Measured by Spinning-Disk Interferometry on the BioCD
Spinning-disc interferometry (SDI) is a high-speed laser scanning approach to surface metrology that uses common-path interferometry to measure protein spots on a BioCD disk. The measurement sensitivity depends on the scanning pitch and on the time-base. Based on high-resolution laser scanning images of printed antibody spots, we quantify the protein sensitivity as a function of the scan parame...
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High speed interferometry on a spinning disk opens new opportunities for sensitive material metrology. One promising application is fast molecular screening in diagnostic medicine.
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A gold ridge microstructure fabricated to a height of lambda/8 on a high-reflectivity substrate behaves as a wave-front-splitting self-referencing interferometer in phase quadrature when illuminated by a Gaussian laser beam and observed in the far field along the optic axis. When immuno-gammaglobulin (IgG) antibodies are selectively immobilized on the gold microstructure, they recognize and bin...
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ژورنال
عنوان ژورنال: Micromachines
سال: 2016
ISSN: 2072-666X
DOI: 10.3390/mi7020031